DNA immunization is an alternative method to develop antibodies. The technique uses an eukaryotic expression vector containing the DNA sequence of your antigene of interest. This plasmid DNA is injected within the muscle of the animal. The cells receiving the DNA express then the antigen of interest and induce an immune response. This allows the possibility to immunize an animal without having to produce or purify the antigen.
The main problem of such techniques resides in the fact that the antigen is generally expressed inside the cells and only presented to the immune system as epitopes. This often reduces the immunogenicity of the antigen and also limits the possibility to generate conformational antibodies necessary for example for in vivo study.
By developing the Staby®boost technology we circumvent this problem and allow a immune response against the full lenght antigen in its conformational status.
The plasmid DNA construct we develop generates a fusion protein between your antigen of interest and a booster sequence. The Booster is a molecular anchor that crosses the cell membrane and pushes the antigen at the cell surface in direct contact with the immune system.
We combine this technology with in vivo electroporation in order to facilitate the entry of the plasmid DNA within the cell and increase the number of cells expressing the fusion protein at their surface.
The service is provided in 3 steps:
- Sequence analysis, strategy/choice of the antigen, genetic construct and in vitro testing by transient transfection of the fonctionnality of the construct (presence of the booster at the cell surface)
- DNA preparation, immunization and test by FACS analysis for the production of antibody against the antigen of interest
- Monoclonal antibody development from spleen and lymph nodes cells.
Using this technique we are able to provide conformational antibody development without having to produce or purify the antigen/protein of interest and even in certain cases for self-antigen. This is an alternative method to obtain antibodies for hard to produce antigen, (i.e membrane proteins), but also against peptides