Despite their immortalized quality, hybridomas can be lost or can lose their specificity to produce your antibody. It is necessary to secure at least in silico your antibody sequence and avoid the loss of several months of work.
Delphi Genetics has developed and proposes a service of antibody sequencing.
We set up a panel of degenerated primers ensuring the amplification of the variable regions from your hybridoma extracted mRNA. By linking this amplification to our Staby®cloning technology, we strongly increase the chance to specifically amplify and clone the 2 chains of your antibody.
After a complete sequencing and sequence validation, we can provide you this sequence or develop an expression vector to produce your antibody or a part of it (Fab, ScFv, ...).