APOL1 variants change C-terminal conformational dynamics and binding to SNARE protein VAMP8
Antibiotic free selection for the high level biosynthesis of a silk-elastin-like protein
Accelerated nucleation of hydroxyapatite using an engineered hydrophobin fusion protein
Protein production without antibiotics (GEN OCT, 2011)
Published in GEN, vol31, n°18, October 2011.
Making Antibiotics Obsolete in Fermentation (GEN august 08)
Alternatives to Mammalian Expression
During Peptalk 2009 conference, participants explored advances in a number of different expression systems including bacterial, yeast, mammalian cells and cell-free systems. The StabyTM technology of Delphi Genetics is described by Dr K. John Morrow in this article (GEN, February,1, 2009) as an alternative to the use of antibiotics.
The art of selective killing: Plasmid toxin/antitoxin systems and their technological applications (Biotechniques 2008, vol45, n°3, 432-4)
Integrated development of up- and downstream processes supported by the Cherry-Tag™ for real-time tracking of stability and solubility of proteins
In this paper, the authors describe the advantages of the use of the Cherry(R) tag developed by Delphi Genetics for protein production and purification. For more information, the article is available here
The Cherry(R)-Tag is presented as a quantitative real-time and in-line product tracking tool which is not fluorescence based.
The tag allowed for distinction between insoluble/non-native proteins and soluble/native species shown in a glutathione-S-transferase (GST) case study.
In upstream processing applications the Cherry(R)-Tag was capable of identifying process conditions of inclusion body formation.
In downstream processing applications it was shown that
Overproduction of the pigment from Azoarcus using Cherry tag
Identification and Biosynthesis of a Novel Xanthomonadin-Dialkylresorcinol-Hybrid from Azoarcus sp. BH72: In this paper of Schöner et al., the authors used the Cherry tag for overproduction of gene clusters encoding the biosynthesis of arcuflavin, a pigment from Azoarcus.
High-throughput micro‐scale cultivations and chromatography modeling: Powerful tools for integrated process development
In this paper, the authors describe methods to develop high-throughput micro-scale cultivations to define the best way to produce recombinant proteins. The Cherry tag was used as a model protein. Proteins were produced using StabyExpress system and SE1 bacteria.
For more information, the paper is available here
Web page about the use of Cherry Express kit
CherryExpress combination with hydrophobic interaction chromatogfraphy
In the article entilted “Influence of Binding pH and Protein Solubility on the Dynamic Binding Capacity in Hydrophobic Interaction Chromatography“, the authors produce proteins using CherryExpress system and purify it using HIC columns.
Use of CherryCodon and StabySwitch to solubilize proteins
http://www.researchtool.jp/modules/tinyd0/index.php?id=15 : webpage (in Japanese) describing the use of CherryCodon and StabySwitch to solubilize proteins in E. coli. An English version is downloadable as an application note.