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The new DNAVAC research project targets the removal of antibiotics in veterinary DNA-vaccines
The new DNAVAC research project targets the removal of antibiotics in veterinary

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Delphi Genetics participation to the AMERE study granted by the European Space Agency (ESA)
Nivelles, Belgium, 23rd of January 2012 – Lambda-X, Delphi Genetics, The d

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Delphi Genetics at Peptalk 2012
Delphi Genetics will be present at the Peptalk meeting taking place in San

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Delphi Genetics announces that all the bacterial strains present in its research kits are completely
Yokohama, Japan, 16 of December 2011 - Delphi Genetics announced today during a

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You are here : Support > Publications

Publications

Latest Delphi Genetics’ Publications and Product Citations.

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CherryCodon

Use of CherryCodon and StabySwitch to solubilize proteins

http://www.researchtool.jp/modules/tinyd0/index.php?id=15 : webpage (in Japanese) describing the use of CherryCodon and StabySwitch to solubilize proteins in E. coli. An English version is downloadable as an application note.

download  download

CherryExpress

Web page about the use of Cherry Express kit

http://bitesizebio.com/2007/09/24/protein-expression-with-a-cherry-on-top/

General

Making Antibiotics Obsolete in Fermentation (GEN august 08)

www.genengnews.com/articles/chtitem.aspx

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The art of selective killing: Plasmid toxin/antitoxin systems and their technological applications (Biotechniques 2008, vol45, n°3, 432-4)
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Alternatives to Mammalian Expression

During Peptalk 2009 conference, participants explored advances in a number of different expression systems including bacterial, yeast, mammalian cells and cell-free systems. The StabyTM technology of Delphi Genetics is described by Dr K. John Morrow in this article (GEN, February,1, 2009) as an alternative to the use of antibiotics.

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Protein production without antibiotics (GEN OCT, 2011)

Published in GEN, vol31, n°18, October 2011.

http://www.genengnews.com/gen-articles/protein-bioproduction-without-antibiotics/3891/

 

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StabyCloning

Population structure of an endemic vulnerable species, the Jamaican Boa (Epicrates Subflavus)
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Molecular genetic analysis of a captive-breeding program: the vulnerable endemic Jamaican yellow boa (Conservation Genetics, 2008)
 
http://www.springerlink.com/content/h240l2710r1g3765/fulltext.pdf
 
 
 
A putative new order of methanogenic Archaea inhabiting the human gut, as revealed by molecular analyses of the mcrA gene (Research in Microbiology, 2008)

http://dx.doi.org/10.1016/j.resmic.2008.06.007

 

Hypermethylation of the DPYD promoter region is not a major predictor of severe toxicity in 5-fluorouracil based chemotherapy (2008)
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Controlling Population Evolution in the Laboratory to Evaluate Methods of Historical Inference (2008)
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Characterization of polymorphic microsatellite loci in the ant Crematogaster scutellaris (Conservation Genetics Resources, 2009)

F. Frizzi, G. Santini, C. Natali, G. Chelazzi and C. Ciofi used StabyCloning to isolate and to characterize several microsatellite loci from Crematogaster scutellaris. StabyCloning was shown very efficient to isolate microsatellites compare to several competitor kits (for more information, see the application note).

 

Testing phylogeographic hypotheses in a Euro-Siberian cold-adapted leaf beetle with coalescent simulations (2009)

Mardulyn et al. used the StabyCloning kit to study the genetic diversity of a Euro-Siberian boreo-montane leaf beetle, Gonioctena pallida. The article was published in Evolution in 2009.

StabyCodon

The cell cycle-associated protein kinase WEE1 regulates cell size in relation to endoreduplication in developing tomato fruit

Gonzalez et al., 2007. The Plant Journal, 51, 642-655

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Genome-wide location analysis reveals a role for Sub1 in RNA polymerase III transcription

Tavenet et al., 2009. PNAS, vol 106, n°34, 14265-14270.
www.pnas.org/content/106/34/14265.full

 

 

StabyExpress

Antibiotic-free selection in E. coli: new considerations for optimal design and improved production

The team of Sanofi Pasteur describes in this paper the use and the efficiency of the StabyExpress technology.  The results show that the presence of an antibiotic resistance gene can indirectly reduce the amount of expressed protein. Since even in absence of selection pressure the gene would be transcribed and account for an additional stress for the host during the fermentation process. StabyExpress allows higher yield without antibiotics.

Engineered recombinant human paraoxonase 1 (PNAS, 2008)

http://www.pnas.org/content/105/35/12780.full

A hypoallergenic variant of Der p 1 as a candidate for mite allergy vaccines (2009)

D. Walgraffe et al. used the StabyExpress kit to produce the ProDer p 1 protein in E. coli and to use it as a candidate for mite allergy vaccines. The article was published in Journal of Allergy and Clinical Immunology in 2009.

StabySwitch

Arizona state university: course about recombinant protein expression

http://photoscience.la.asu.edu/photosyn/courses/BIO_343/lab/Experiment-I.html