High-efficiency competent DG1 E. coli cells are supplied in a 96-tube tray (50µl of competent bacteria in each tube). This convenient format gives you the freedom to perform from 1 to 96 transformations at a time, without thawing more tubes of cells than needed. You can remove individual tubes by cutting cap strips. Thus, this format eliminates the reduced efficiencies that occur as a result of repeated freezing and thawing and makes possible high-throughput transformation. Racks feature 8 x 12 holes pattern for rapid processing using multi-channel pipettors. Alpha-numeric, non-reversing, rack and lid ensure positive sample ID.

DG1 E. coli cells are offered in the 24, 48 and 96 formats, chemically-competent, so you can pick the one that best suits the needs of your experiment. The competent E. coli bacteria are consistently high quality, with high efficiencies (>1x10⁹ cfu/µg monomer plasmid DNA). The competent bacteria come complete with SOC media. Each tube contains 50µl of competent E. coli, which is sufficient for one high efficiency transformation.

DG1 is convenient for use in cloning, plasmid preparation and library construction. It is endonuclease and recombination minus. It allows lacZalpha complementation for blue/white screening.

DG1 electro-competent bacteria are available on request.

Genotype:
DG1: mcrA D(mrr-hsdRMS-mcrBC, modification-, restriction-) F80lacZDM15 DlacX74 recA1 araD139 D(ara-leu)7697 galU galK rpsL endA1 nupG

Benefits:
-Freedom to use anywhere from 1-96 reactions
-No wasted reactions
-High efficiency (>10⁹ cfu/µg)
-Economical pricing
-Convenient for high-throughput use