Staby^TMSwitch is an auto-inducible medium: metabolization of medium components during bacterial growth will automatically induce protein expression at high cell density. Thus, it is neither necessary to add IPTG (isopropyl -β-D-thiogalactoside) nor to monitor optical density during bacterial growth. Furthermore, the target protein yield is often higher than using conventional IPTG induction. When using an autoinducible medium, it is essential to completely stabilize the plasmid encoding the protein of interest to obtain high expression yield. It is thus strongly recommended to use Staby^TM products (StabyExpress^TM, Staby^TMCodon, Cherry^TMExpress or Cherry^TMCodon) for high-yield protein expression. As illustrated on the figure, it is easier and faster to use Staby^TM Swicth than IPTG.

Results

The Staby^TMSwitch medium is convenient for growing multiple cultures in parallel. Rapid growth to high densities is achieved (for maximizing the yield and efficiency of protein production) without the need to monitor bacterial growth nor to add inducer. As illustrated on the figure, expression yield is similar or higher using Staby^TMSwitch than using conventional IPTG induction. Moreover, some proteins exhibit better conformation and solubility when using Staby^TMSwitch instead of IPTG.

Content

The Staby^TMSwitch auto-inducible medium is a complete culture medium supplied as a sterile, ready-to-use solution. Each box contains two bottles of 1 liter of cultutre medium. The sterile medium is stable for several months when stored at room temperature or 4°C.

 Benefits of the StabySwitch medium